期刊
FEBS LETTERS
卷 486, 期 2, 页码 155-158出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02266-3
关键词
phosphate starvation; inorganic pyrophosphate; proton pump; vacuolar type H(+)-pyrophosphatase; vacuolar type H(+)-ATPase; Brassica napus
The influence of phosphate (Pi) deprivation on the vacuolar H(+)-translocating pyrophosphatase (PPiase) and ATPase in tonoplast vesicles from Brassica napus suspension cells was assessed. Pi starvation significantly elevated the ratios of PPi-: ATP-dependent H(+) translocation rate and H(+)-PPiase: H(+)-ATPase hydrolytic activities. These increases were reversed 36 h following resupply of 2.5 mM Pi to the Pi-starved cells. Immunoblotting indicated that Pi starvation also induced a two-fold increase in the amount of H(+)-PPiase protein, whereas the amount of H(+)-ATPase remained unchanged. It is proposed that H(+)-PPiase facilitates the conservation of limited ATP pools, and Pi recycling during Pi stress. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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