期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
卷 1517, 期 1, 页码 159-163出版社
ELSEVIER
DOI: 10.1016/S0167-4781(00)00240-2
关键词
1-deoxy-D-xylulose 5-phosphate reductoisomerase; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; monoterpenoid indole alkaloid; 2C-methyl-D-erythritol 4-phosphate pathway; Catharanthus roseus
Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to l-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.
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