期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 279, 期 2, 页码 615-620出版社
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2000.4008
关键词
human histamine H4 receptor; HH4R; histamine; G protein-coupled receptor; GPCR; histamine receptor; peripheral tissues; orphan GPCR; oGPCR; database search
A new histamine receptor, HH4R, was cloned from human leukocyte cDNA. The deduced amino acid sequence showed about 40% identity to that of the human histamine H3 receptor, HH3R. HH4R-expressing cells responded to histamine, inhibiting forskolin-induced cAMP accumulation. An H3 agonist, N-alpha -methylhistamine (NAMHA), bound specifically to HH4R, while another H3 agonist, R(-)-alpha -methylhistamine (RAMHA), and the H3 antagonist, thioperamide, competed with this binding. RAMHA,NAMHA, and imetit inhibited forskolin-induced cAMP accumulation in HH4R-expressing cells. However, the binding affinities and agonistic activities of H3 agonists to HH4R were weaker than those to HH3R Low expression of HH4R was detected in a wide variety of peripheral tissues by RT-PCR; however, in contrast with HH3R, expression was not detected in the brain. These observations indicate that the clone is a distinct histamine receptor from HH3R, and thus is named HH4R. (C) 2000 Academic Press.
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