期刊
FEBS LETTERS
卷 487, 期 1, 页码 71-75出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02283-3
关键词
intron; retrotransposon; genomic library; tRNA; synteny; Saccharomyces cerevisiae
As part of the comparative genomics project 'GENOLEVURES', we studied the Kluyveromyces marxianus var. marxianus strain CBS712 using a partial random sequencing strategy. With a 0.2 x genome equivalent coverage, me identified ca, 1300 novel genes encoding proteins, some containing spliceosomal introns with consensus splice sites identical to those of Saccharomyces cervisiae, 28 tRNA genes, the whole rDNA repeat, and retrotransposons of the Ty1/2 family of S.cervisiae with diverged Long Terminal Repeats. Functional classification of the K. marxianus genes, as well as the analysis of the paralogous gene families revealed few differences with respect to S. cervisiae. Only 42 K, marxianus identified genes are without detectable homolog in the baker's yeast. However, we identified several genetic rearrangements between these two yeast species. (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
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