期刊
FEBS LETTERS
卷 487, 期 2, 页码 156-160出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02337-1
关键词
cysteine proteinase; cysteine proteinase inhibitor; cathepsin; cystatin; stopped-flow kinetics
Cystatins A and C were both shown to inhibit cathepsin B by a two-step mechanism, involving an initial weak interaction followed by a conformational change. Disruption of the major salt bridge anchoring the occluding loop of cathepsin B to the main body of the enzyme by mutation of His110 to Ala converted the binding to an apparent one-step reaction, The second step of cystatin binding to cathepsin B must therefore be due to the inhibitor having to alter the conformation of the enzyme by displacing the occluding loop to allow a tight complex to be formed. Cystatin A mas appreciably less effective in displacing the loop than cystatin C, resulting in a considerably lower overall inhibition rate constant. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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