期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 28, 期 1, 页码 70-80出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(00)00277-5
关键词
Aspergillus niger; Pycnoporus cinnabarinus; sugar beet pulp; maize bran; induction; polysaccharide-degrading enzymes; feruloyl esterases
The filamentous fungal strains Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. A large spectrum of polysaccharide-degrading enzymes was produced by A. niger and very few levels of feruloyl esterases were found. In contrast, P, cinnabarinus culture filtrate contained low amount of polysaccharide-degrading enzymes and no feruloyl esterases. In order to enhance feruloyl esterases in A. niger cultures, feruloylated oligosaccharide-rich fractions were prepared from sugar beet pulp or cereal bran and used as carbon sources. Number of polysaccharide-degrading enzymes were induced. Feruloyl esterases were much higher in maize bran-based medium than in sugar beet pulp-based medium, demonstrating the ability of carbon sources originating from maize to induce the synthesis of feruloyl esterases. Thus, A. niger I-1472 could be interesting to release ferulic acid from sugar beet pulp or maize bran. (C) 2001 Elsevier Science Inc. AU rights reserved.
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