期刊
ONCOGENE
卷 20, 期 2, 页码 141-146出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1204077
关键词
oncogenic transformation; differential gene expression; DNA micro-array; tumor suppressor
资金
- NCI NIH HHS [CA42564] Funding Source: Medline
- NIDDK NIH HHS [5 T23 DK 07022] Funding Source: Medline
Line 10T1/2 mouse fibroblast overexpressing the v-Jun oncoprotein mere morphologically altered, grew into multilayered foci in culture and formed colonies when suspended in agar, The growth rate of the v-Jun-transformed 10T1/2 cells was not changed significantly from that of the untransformed parental cells, but the saturation density of the transformed cultures exceeded that of normal controls by a factor of 2, mRNA extracted from v-Jun-transformed 10T1/2 cells was analysed for differential gene expression with DNA micro-array technology. One of the targets downregulated by v-Jun was identified as SSeCKS (Src-suppressed C kinase substrate). Re-expression of SSeCKS in v-Jun-transformed fibroblasts reversed the transformed phenotype of the cells. Their ability to form foci was reduced to background levels, the number and size of agar colonies was lowered by a factor of 10 and the saturation density was significantly diminished. However, expression of SSeCKS had little effect on the morphology of v-Jun-transformed 10T1/2 cells. These data suggest that the SSeCKS protein has growth-attenuating properties. Down-regulation of SSeCKS may be essential for Jun-induced transformation.
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