期刊
LIFE SCIENCES
卷 68, 期 8, 页码 921-931出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0024-3205(00)00999-1
关键词
flavonoids; macrophages; nitric oxide; inducible nitric oxide synthase; prostaglandin E-2; cyclooxygenase-2
The present study focuses on the effect of various naturally occurring flavonoids (apigenin, galangin, morin, naringenin, quercetin, and silymarin) on nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production induced by lipopolysaccharide (LPS) in the macrophage cell line J774A.1. Moreover, we evaluated flavonoid modulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) enzyme expression by western blot analysis. Apigenin and quercetin (0.5-50 muM) were the most potent inhibitors of NO production and this effect was concentration-dependent and significant at 5 and 50 muM. These data were consistent with the modulation of iNOS enzyme expression. A similar pattern was observed considering the inhibitory effect of flavonoids on LPS-induced PGE, release and COX-2 expression. Quercetin, galangin, apigenin, and naringenin markedly decreased PGE, release and COX-2 expression in a concentration-dependent manner. This study suggests that inhibition of iNOS and COX-2 expression by flavonoids may be one of the mechanisms responsible for their anti inflammatory effects. (C) 2001 Elsevier Science Inc. All rights reserved.
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