期刊
FEBS LETTERS
卷 488, 期 1-2, 页码 29-33出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02400-5
关键词
AVP2/AVPL1 gene; Golgi apparatus; green fluorescent protein; brefeldin A; H+-PPase; Arabidopsis thaliana
Vacuolar H+-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigated the tissue distribution and the subcellular localization, respectively, of a novel type H+-PPase encoded by AVP2/AVPL1 identified in the Arabidopsis thaliana genome. We showed that AVP2/AVPL1 is highly expressed at the trichoma and the filament of stamen. Furthermore, the fluorescence of GFP-tagged AVP2/AVPL1 shelved small dotlike structures that were observed throughout the cytoplasm of various Arabidopsis cells under a fluorescent microscope. The distribution of this dot-like fluorescent pattern was apparently affected by a treatment with brefeldin A. Moreover, we demonstrated that most dot-like fluorescent structures colocalized with a Golgi resident protein. These findings suggest that this novel type H+-PPase resides on the Golgi apparatus rather than the vacuolar membrane. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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