期刊
JOURNAL OF CHROMATOGRAPHY A
卷 907, 期 1-2, 页码 145-154出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)01041-4
关键词
preparative chromatography; membranes; breakthrough volumes; antibodies
The large-scale production of recombinant monoclonal antibodies demands economical purification processes with high throughputs. The potential for ion-exchange membrane adsorbers to replace traditional ion-exchange columns was evaluated. Breakthrough capacities of commercially available cation-exchange membranes were determined as a function of Row-rate and layer number. Due to economic and process restrictions, cation-exchange membranes may not currently be advantageous for process-scale antibody purification in a bind and elute mode. However, anion-exchange membranes in a how-through mode may provide a reasonable alternative to columns for the removal of low levels of impurities such as DNA, host cell protein, and virus. (C) 2001 Elsevier Science B.V. All rights reserved.
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