期刊
JOURNAL OF CHROMATOGRAPHY A
卷 907, 期 1-2, 页码 343-346出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)00960-2
关键词
counter-current chromatography; Polygonum cuspidatum; plant material; resveratrol; piceid; polyphenols
High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of resveratrol and piceid from the dried roots (20.0 g) of Polygonum cuspidatium. The EtOAc extracts were separated with chloroform-methanol-water (4:3:2, v/v). Resveratrol was identified in fraction 5. The water extracts were separated first with EtOAc-EtOH-water (10:1:10, v/v) and then with the same solvent system at the modified volume ratio of 70:1:70. Yields of resveratrol and piceid obtained were 2.18% and 1.07%. Chemical structures of the purified resveratrol and piceid were confirmed by electrospray ionization MS and H-1 nuclear magnetic resonance spectroscopy. (C) 2001 Elsevier Science B.V. All rights reserved.
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