期刊
BIOCHIMIE
卷 103, 期 -, 页码 80-88出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2014.04.006
关键词
hRPA; G-quadruplexes; G4 ligands; Telomeres; Photo-crosslinking
资金
- Ligue Nationale contre le Cancer, Equipe Labellisee
- Agence Nationale de la Recherche [ANR-09-BLAN-0355]
- Institut National du Cancer [TP53-INTRON3]
- RFBR [12-04-00487-a]
- Ligue Nationale contre le Cancer
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0355] Funding Source: Agence Nationale de la Recherche (ANR)
Replication protein A (RPA) is a single-stranded DNA binding protein that plays an essential role in telomere maintenance. RPA binds to and unfolds G-quadruplex (G4) structures formed in telomeric DNA, thus facilitating lagging strand DNA replication and telomerase activity. To investigate the effect of G4 stability on the interactions with human RPA (hRPA), we used a combination of biochemical and biophysical approaches. Our data revealed an inverse relationship between G4 stability and ability of hRPA to bind to telomeric DNA; notably small G4 ligands that enhance G4 stability strongly impaired G4 unfolding by hRPA. To gain more insight into the mechanism of binding and unfolding of telomeric G4 structures by RPA, we carried out photo-crosslinking experiments to elucidate the spatial arrangement of the RPA subunits along the DNA strands. Our results showed that RPA1 and RPA2 are arranged from 5' to 3' along the unfolded telomeric G4, as already described for unstructured single-stranded DNA, while no contact is possible with RPA3 on this short oligonucleotide. In addition, these data are compatible with a 5' to 3' directionality in G4 unfolding by hRPA. (C) 2014 Elsevier Masson SAS. All rights reserved.
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