期刊
FEBS LETTERS
卷 488, 期 3, 页码 116-122出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02373-5
关键词
human glutaminase; brain; two-hybrid; PDZ protein; snytrophin
The enzyme glutaminase in brain is responsible for the synthesis of neurotransmitter glutamate, We used the two-hybrid genetic selection system in yeast to look for interactors of glutaminase in human brain. We have identified two proteins containing PDZ domains, alpha1-syntrophin and a glutaminase-interacting protein, named GIP, that showed association with human glutaminase L, as deduced from specificity test of the two-hybrid system. The complete GIP cDNA clone has 1315 nucleotides with a 372-base open reading frame encoding a 124-amino acids protein. Glutaminase associates with both PDZ proteins through its C-terminal end; mutagenesis of single amino acids revealed the sequence -ESXV as essential for the interaction. These data suggest the possibility that PDZ domain-containing proteins are in, ol, ed in the regulation of glutaminase in brain. (C) 2001 Federation of European Biochemical Societies.
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