期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 11, 期 4-6, 页码 765-769出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1381-1177(00)00022-9
关键词
chloroperoxidase; random mutagenesis; sulfoxidation; organic solvents; immobilisation
Random mutagenesis of Caldariomyces fumago was carried out to produce a chloroperoxidase (CPO) with enhanced activity in mixtures of aqueous buffers and organic cosolvents. A mutant CPO with a 3,4-fold increased activity in 40% aqueous tert-butyl alcohol was obtained. A CPO-surfactant conjugate, prepared by colyophilisation, mediated the oxidation of thioanisole to its (R)-sulfoxide by TBHP in water-saturated isooctane, Copolymerisation of CPO and a toluenediisocyanate prepolymer resulted in a stable preparation that mediated the oxidation of indole to 2-oxindole in a range of organic solvents. The highest yield was obtained in 1-octanol. (C) 2001 Elsevier Science B.V. All rights reserved.
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