期刊
BIOCHIMIE
卷 93, 期 3, 页码 389-398出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2010.11.005
关键词
IFN; PML; Stat; Nuclear bodies; SUMO
资金
- Centre National de la Recherche Scientifique
- Ligue Nationale Contre le Cancer
PML, also known as TRIM19, belongs to the family encoding a characteristic RBCCrIRIM motif comprising several cysteine-rich zinc-binding domains (RING and B-boxes) and a coiled-coil domain. The RBCC domain and the covalent modification of PML by the small ubiquitin-like modifier (SUMO) are required for PML localization within the nuclear bodies (NBs). Analysis of PML-/- mice provided evidence for a physiological role of PML in apoptosis. Cells derived from these mice are defective in the induction of apoptosis by interferon (IFN). PML is expressed as a family of cytoplasmic and nuclear isoforms (PML I-VII) as a result of alternative splicing. Herein, we show that overexpression of all nuclear PML isoforms (I-VI) in human cells increased IFN gamma-induced STAT1 phosphorylation, resulting in higher binding of STAT1 to DNA, higher activation of IFN-stimulated genes (ISGs), and an increase in the expression of their products. These effects, observed with IFN gamma and not IFN alpha, required PML localization in the nucleus as they were not observed with the cytoplasmic isoform PMLVIIb or the cytoplasmic variants of PMLIV. They also necessitated PML SUMOylation and its RING finger domain. Conversely, downregulation of PML by RNA interference was accompanied by decrease in IFN gamma-induced STAT1 phosphorylation, STAT1 DNA binding, transcription of ISGs and in the expression of their products. In addition, IFN gamma-mediated STAT1 DNA-binding activity was decreased in PML-/- MEFs compared with wild-type MEFs. Taken together these results demonstrate that PML functions as a positive regulator of IFN gamma signaling. (C) 2010 Elsevier Masson SAS. All rights reserved.
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