期刊
FEBS LETTERS
卷 489, 期 1, 页码 101-106出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02076-2
关键词
gastrin; cholecystokinin; rat-1 cell; protein kinase C; protein kinase D
资金
- NIDDK NIH HHS [DK 55003, DK 17294] Funding Source: Medline
Addition of gastrin or cholecystokinin octapeptide (CCK-8) to cultures of Rat-1 cells stably transfected with the CCK2 (CCKB/gastrin) receptor induced protein kinase D (PKD) activation that was detectable within 1 min and reached a maximum (similar to 10-fold) after 2.5 min of hormonal stimulation. Half-maximal PKD activation for both CCK-8 and gastrin mas achieved at 10 nM. Treatment with various concentrations of the selective PKC inhibitors Ro 31-8220 or GF-I potently blocked PKD activation induced by subsequent addition of CCK-8 in a concentration-dependent fashion. Our results indicate that PKC-dependent PKD activation is a novel early event in the action of gastrin and CCK-8 via CCK2 receptors. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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