期刊
BIOCHIMIE
卷 92, 期 6, 页码 665-675出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2010.04.006
关键词
Reproduction; Ovulation; Prostanoid; Gonadotropin; Gene expression profile
资金
- Japan Society for the Promotion of Science for Young Scientists
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Ministry of Health and Labor of Japan
- National Institute of Biomedical Innovation of Japan
- Mochida Memorial Foundation
- Naito Foundation
To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2(-/-) cumuli before and after ovulation by using microarrays. We prepared cumulus cells from mice just before and 3, 9 and 14 h after human chorionic gonadotropin injection. Key genes including cAMP-related and epidermal growth factor (EGF) genes, as well as extracellular matrix-(ECM-) related and chemokine genes were up-regulated in WT cumuli at 3 h and 14 h, respectively. Ptger2 deficiency differently affected the expression of many of the key genes at 3 h and 14 h. These results indicate that the gene expression profile of cumulus cells greatly differs before and after ovulation, and in each situation, PGE(2)-EP2 signaling plays a critical role in cAMP-regulated gene expression in the cumulus cells under physiological conditions. (C) 2010 Published by Elsevier Masson SAS.
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