期刊
BIOCHIMIE
卷 91, 期 4, 页码 475-483出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2008.12.001
关键词
SBDS ortholog; Trypanosoma cruzi; RNA-protein interaction; Protein structure
资金
- FAPESP [CBME/CEPID-98/14138-2, SMoIBNet-00/10266-8, 06/02083-7, 02/12597-7]
- FAPESP
The human SBDS gene and its yeast ortholog SDOI encode essential proteins that are involved in ribosome biosynthesis. SDOI has been implicated in recycling of the ribosomal biogenesis factor Tif6p from pre-66S particles as well as in translation activation of 60S ribosomes. The SBDS protein is highly conserved, containing approximately 250 amino acid residues in animals, fungi and Archaea, while SBDS orthologs of plants and a group of protists contain an extended C-terminal region. In this work, we describe the characterization of the Trypanosoma cruzi SBDS ortholog (TcSBDS). TcSBDS co-fractionates with polysomes in sucrose density gradients, which is consistent with a role in ribosome biosynthesis. We show that TcSBDS contains a C-terminal extension of 200 amino acids that displays the features of intrinsically disordered proteins as determined by proteolytic, circular dichroism and NMR analyses. Interestingly, the C-terminal extension is responsible for TcSBDS-RNA interaction activity in electrophoretic mobility shift assays. This finding suggests that Trypanosomatidae and possibly also other organisms containing SBDS with extended C-terminal regions have evolved an additional function for SBDS in ribosome biogenesis. (C) 2008 Elsevier Masson SAS. All rights reserved.
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