期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 530, 期 3, 页码 395-403出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1111/j.1469-7793.2001.0395k.x
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1. We have studied the effect of bradykinin (Bk) on fibroblast-like satellite (FLS) cells isolated from cultures of neonatal rat dorsal root ganglia (DRG). 2. In voltage-clamped FLS cells Bk evoked an inward current response that was concentration dependent with a half-maximal concentration of 2 nM. 3. In indo-1 AM-loaded FLS cells Bk evoked a rise in intracellular Ca2+ that was concentration dependent with a half-maximal concentration of 1 nM. 4. The FLS cells still, produced an inward current in response to Bk in the absence of extracellular Ca2+ but the response was inhibited if the intracellular concentration of EGTA was increased from 0.5 to 5 mM, which suggests that the inward current was dependent on the release and subsequent rise of intracellular Ca2+. 5. The reversal potential of the Bk-induced inward current was consistent with the current being due to an increase in Cl- conductance and shifted in a Nernstian manner when the intracellular Cl- concentration was reduced. 6. The inward current response to Bk was blocked by the B-2 receptor antagonist HOE-140, which indicates that the response was due to activation of B-2 receptors. 7. The data suggest that Bk evokes a rise in intracellular Ca2+ and activation of a Ca2+-activated Cl- conductance in the FLS cells and raise the possibility that FLS cells contribute to the proinflammatory effects of Bk in DRG.
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