期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1844, 期 9, 页码 1694-1707出版社
ELSEVIER
DOI: 10.1016/j.bbapap.2014.06.018
关键词
Cantareus aspersus metallothionein; Cadmium; Copper; Sequence determinant
资金
- Spanish Ministerio de Economia y Competitividad [BIO2012-39682-C02-01, BIO2012-39682-C02-02]
- Austrian Science Foundation [P 23635-B20]
- Acciones Integradas (Austria) [HU2006-0027, ES 02/2007]
- Departament de Quimica, Universitat Autanoma de Barcelona
- Austrian Science Fund (FWF) [P 23635] Funding Source: researchfish
- Austrian Science Fund (FWF) [P23635, I1482] Funding Source: Austrian Science Fund (FWF)
In Proteomics, gene/protein families including both specialized and non-specialized paralogs are an invaluable tool to study the evolution of structure/function relationships in proteins. Metallothioneins (MTs) of the pulmonate gastropod molluscs (snails) offer one of the best materials to study the metal-binding specificity of proteins, because they consist of a polymorphic system that includes members with extremely distinct metal preferences but with a high protein sequence similarity. Cantareus aspersus was the first snail where three paralogous MTs were isolated: the highly specific cadmium (CaCdMT) and copper (CaCuMT) isoforms, and an unspecific CaCd/CuMT isoform, so called because it was natively isolated as a mixed Cd and Cu complex. In this work, we have thoroughly analyzed the Zn2+-, Cd2+- and Cu+-binding abilities of these three CaMTs by means of the spectroscopic and spectrometric characterization of the respective recombinant, as well as in vitro-substituted, metal-complexes. The comparison with the orthologous HpMTs and the study of the isoform-determinant residues allow correlating the protein sequence variability with the coordination capabilities of these MTs. Surprisingly, the CaCuMT isoform exhibits a stronger Cu-thionein character than the HpCuMT ortholog, and the CaCd/CuMT isoform could be defined as a non-optimized Cu-thionein, which has not attained any defined functional differentiation in the framework of the snail MT gene/protein family. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据