期刊
CARDIOVASCULAR RESEARCH
卷 49, 期 2, 页码 298-307出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0008-6363(00)00256-X
关键词
Ca-channel; e-c coupling; heart failure; myocytes; remodeling
资金
- NHLBI NIH HHS [R01 HL61537, R01 HL61534] Funding Source: Medline
- NIAMS NIH HHS [R01 AR46448] Funding Source: Medline
Objective: Persistent supraventricular tachycardia leads to the development of a dilated cardiomyopathy with impairment of excitation-contraction (EC) coupling. Since the initial trigger for EC coupling in ventricular muscle is the influx of Ca2+ through L-type Ca2+ channels (I-Ca) in the transverse tubules (T-tubules), we determined if the density of the T-tubule system and L-type Ca2+ channels change in canine tachycardia pacing-induced cardiomyopathy. Methods: Confocal imaging of isolated ventricular myocyles stained with the membrane dye Di-8-ANEPPS was used to image the T-tubule system, and standard whole-cell patch clamp techniques were used to measure I-Ca and intramembrane charge movement. Results: A complex staining pattern of interconnected tubules including prominent transverse components spaced every similar to1.6 mum was present in control ventricular myocytes, but failing cells demonstrated a far less regular T-tubule system with a relative loss of T-tubules. In confocal optical slices, the average % of the total cell area staining for T-tubules decreased from 11.5+/-0.4 in control to 8.7+/-0.4% in failing cells (P<0.001). Whole-cell patch clamp studies revealed that I-Ca density was unchanged. Since whole-cell I-Ca is due to both the number of channels as well as the functional properties of those channels, we measured intramembrane charge movement as an assay for changes in channel number. The saturating amount of charge that moves due to gating of L-type Ca2+ channels, Q(on,max). was decreased from 6.5+/-0.6 in control to 2.8+/-0.3 fC/pF in failing myocytes,(P<0.001). Conclusions: Cellular remodeling in heart failure results in decreased density of T-tubules and L-type Ca2+ channels, which contribute abnormal EC coupling. (C) 2001 Elsevier Science B.V. All rights reserved.
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