期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1834, 期 2, 页码 583-592出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2012.11.004
关键词
Partitioning; Protein structure; Aqueous two-phase system; Structural change; Solvent interaction analysis
Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired. (C) 2012 Elsevier B.V. All rights reserved.
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