4.3 Article

High throughput characterization of structural differences between closely related proteins in solution

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ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbapap.2012.11.004

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Partitioning; Protein structure; Aqueous two-phase system; Structural change; Solvent interaction analysis

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Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired. (C) 2012 Elsevier B.V. All rights reserved.

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