Bioenergetic consequences of opening the ATP-sensitive K+ channel of heart mitochondria

There is an emerging consensus that pharmacological opening of the mitochondrial ATP-sensitive K+ (K-ATP) channel protects the heart against ischemia-reperfusion damage; however, there are widely divergent views on the effects of openers on isolated heart mitochondria. We have examined the effects of diazoxide and pinacidil on the bioenergetic properties of rat heart mitochondria. As expected of hydrophobic compounds, these drugs have toxic, as well as pharmacological, effects on mitochondria. Both drugs inhibit respiration and increase membrane proton permeability as a function of concentration, causing a decrease in mitochondrial membrane potential and a consequent decrease in Ca2+ uptake, but these effects are not caused by opening mitochondrial K-ATP channels. In pharmacological doses (<50 M), both drugs open mitochondrial K-ATP channels, and resulting changes in membrane potential and respiration are minimal. The increased K+ influx associated with mitochondrial K-ATP channel opening is similar to 30 nmol.min(-1).mg(-1), a very low rate that will depolarize by only 1-2 mV. However, this increase in K+ influx causes a significant increase in matrix volume. The volume increase is sufficient to reverse matrix contraction caused by oxidative phosphorylation and can be observed even when respiration is inhibited and the membrane potential is supported by ATP hydrolysis, conditions expected during ischemia. Thus opening mitochondrial K-ATP channels has little direct effect on respiration, membrane potential, or Ca2+ uptake but has important effects on matrix and intermembrane space volumes.