期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1794, 期 12, 页码 1838-1847出版社
ELSEVIER
DOI: 10.1016/j.bbapap.2009.08.024
关键词
Human (pro)renin receptor; Decoy peptide; Hinge peptide; Renin; Prorenin
资金
- Ministry of Education, Science and Culture of Japan [1907165]
- NIH [H58205]
- Japan Society for the Promotion of Science [2007/2009: P07165]
An important role of the decoy peptide sequence has recently been suggested in vitro for the binding of prorenin to the (pro)renin receptor [(P)RR]. In this study, other prospective crucial regions in renin and prorenin responsible for their interaction with (P)RR were investigated using various kinds of peptides, e.g., the hinge S(149)QGVLKEDVF(158) designed from the structure of renin also common to prorenin, (LPTDTTTF8P)-P-1P, (LPTDTTTFKRIFLKR15P)-P-1P and the decoy ((RIFLKRMPSI19P)-I-10P) designed from the predicted structure of prorenin. For the kinetic analysis, the recombinant h(P)RR was immobilized on the biosensor surface through a specific anti-(P)RR antibody. In case of the equilibrium state analysis, the (P)RR was directly adsorbed on plastic wells for observing the bindings of renin/prorenin. The dissociation constants (K-D) for the bindings of renin and prorenin to the pre-adsorbed receptors were 4.5 and 1.0 nM. respectively, similar to those stated in the kinetic study by BIAcore assay. The hinge region peptide bound to (P)RR in a dose-dependent manner with a K-D estimated 17.0 nM which was five times higher than that of the decoy. The K-D values for (LPTDTTTF8P)-P-1P and (LPTDTTTFKRIFLKR15P)-P-1P were 52 and 7.6 nM, respectively. The hinge peptide, as the decoy, inhibited the bindings of renin and prorenin to (P)RR. The inhibition constant (K-i) for the binding of renin and prorenin by the decoy and hinge were 16.7 and 15.1, and 37.1 and 30.7 nM, respectively. These in vitro studies suggest that renin has a single and prorenin has at least two high affinity binding sites for the (P)RR. (C) 2009 Elsevier B.V. All rights reserved.
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