期刊
BRITISH JOURNAL OF HAEMATOLOGY
卷 112, 期 2, 页码 459-465出版社
WILEY
DOI: 10.1046/j.1365-2141.2001.02534.x
关键词
megakaryocyte; haematopoeisis; thrombopoietin; bone marrow; transendothelial migration
类别
Megakaryocytes (MKs) have been found in the peripheral circulation, suggesting that they can migrate out of the bone marrow. In order to evaluate if megakaryocytic differentiation confers a migratory phenotype, we investigated this property in the haematopoietic cell lines MO7e and UT-7/mpl and in CD34(+) progenitor cells before and after induction of differentiation by thrombopoietin (TPO). Migration was studied using a bicompartmental culture system in the presence or absence of a bone marrow endothelial cell monolayer. Preincubation with TPO led to a significant reduction in stromal cell-derived factor-1 (SDF-1)-induced migration of MO7e cells (0.7% +/- 0.08% for TPO-treated vs. 2.6% +/- 0.3% for controls P < 0.05). A similar decreased migratory response was seen with UT-7/mpl cells (7.4% +/- 0.4% for TPO-treated vs. 11.1% +/- 0.01% for controls, P < 0.05), although these cells did not migrate in response to SDF-1. CD34(+) cells partially differentiated with TPO showed decreased migration following further TPO-induced maturation (13.9% +/- 1.8% for TPO-treated vs. 24.1% +/- 1.8% for untreated, P < 0.05). This reduction was more pronounced in the large MK (greater than or equal to 4n) fraction. These results demonstrate that megakaryocytic differentiation is accompanied by a partial suppression of the haematopoietic cell migratory phenotype.
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