Spectrophotometric determination of ampicillin, dicluxacillin, flucloxacillin and amoxicillin antibiotic drugs: ion-pair formation with molybdenum and thiocyanate

A sensitive spectrophotometric method is developed for the determination of some antibiotic drugs such as ampicillin (amp), dicluxacillin (dicl), flucloxacillin (fluc) and amoxicillin (amox). The method involves the formation of ion-pairs between these drugs under investigation and inorganic complex of Mo (V)-thiocyanate followed by its extraction with methylene chloride. The optimum conditions for the ion-pairs formation are established. The method permits the determination of amp, did, flue and amor over a concentration range of 1.5-77.5, 3-75, 1.5-79 and 7.5-75 mug ml(-1) respectively. The sensitivity (S) is found to be 0.017, 0.061, 0.014 and 0.073 mug cm(-2) for amp, did, flue and amor, respectively. The method is simple, rapid, reproducible and accurate within +/-1%. The method is applicable for the assay of the foul drugs under investigation in different dosage forms and the results are in good agreement with those obtained by the official method. (C) 2001 Elsevier Science B.V. All rights reserved.