期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1813, 期 6, 页码 1220-1229出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2011.03.009
关键词
FTIR microspectroscopy; SN oocytes; NSN oocytes; Oocyte maturation; Developmental competence; Polyadenylation
资金
- Fondazione IRCCS Policlinico San Matte. Pavia (I)
- University of Milano-Bicocca
- FAR (Fondo di Ateneo per la Ricerca)
- University of Milano-Bicocca (I)
Mammalian antral oocytes with a Hoescht-positive DNA ring around the nucleolus (SN) are able to resume meiosis and to fully support the embryonic development, while oocytes with a non-surrounded nucleolus (NSN) cannot. Here, we applied FTIR microspectroscopy to characterize single SN and NSN mouse oocytes in order to try to elucidate some aspects of the mechanisms behind the different chromatin organization that impairs the full development of NSN oocyte-derived embryos. To this aim, oocytes were measured at three different stages of their maturation: just after isolation and classification as SN and NSN oocytes (time 0); after 10 h of in vitro maturation, i.e. at the completion of the metaphase I (time 1); and after 20 h of in vitro maturation, i.e. at the completion of the metaphase II (time 2). Significant spectral differences in the lipid (3050-2800 cm(-1)) and protein (1700-1600 cm(-1)) absorption regions were found between the two types of oocytes and among the different stages of maturation within the same oocyte type. Moreover, dramatic changes in nucleic acid content, concerning mainly the extent of transcription and polyadenylation, were detected in particular between 1000 and 800 cm(-1). The use of the multivariate principal component-linear discriminant analysis (PCA-LDA) enabled us to identify the maturation stage in which the separation between the two types of oocytes took place, finding as the most discriminating wavenumbers those associated to transcriptional activity and polyadenylation, in agreement with the visual analysis of the spectral data. (C) 2011 Elsevier B.V. All rights reserved.
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