期刊
JOURNAL OF BACTERIOLOGY
卷 183, 期 3, 页码 1058-1068出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.183.3.1058-1068.2001
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资金
- NIAID NIH HHS [T32 AI07101, T32 AI007101] Funding Source: Medline
- NIGMS NIH HHS [R01 GM043577, GM43577] Funding Source: Medline
Bacteria with circular chromosomes have evolved systems that ensure multimeric chromosomes, formed by homologous recombination between sister chromosomes during DNA replication, are resolved to monomers prior to cell division. The chromosome dimer resolution profess in Escherichia coli is mediated by two tyrosine family site-specific recombinases, XerC and XerD, and requires septal localization of the division protein FtsK. The Xer recombinases act near the terminus of chromosome replication at a site known as dif (Ecdif). In Bacillus subtilis the RipX and CodV site-specific recombinases have been implicated in an analogous reaction. We present here genetic and biochemical evidence that a 28-bp sequence of DNA (Bsdif), lying 6 degrees counter-clockwise;ise from the B. subtilis terminus of replication (172 degrees), is the site at which RipX and CodV catalyze site-specific recombination reactions required for normal chromosome partitioning. Bsdif in vivo recombination did not require the B. subtilis FtsK homologues, SpoIIIE and YtpT, We also show that the presence or absence of the B. subtilis SPP-bacteriophage, and in particular its yopP gene product, appears to strongly modulate the extent of the partitioning defects seen in codV strains and, to a lesser extent, those seen in ripX and dif strains.
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