期刊
BRAIN RESEARCH PROTOCOLS
卷 6, 期 3, 页码 159-166出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1385-299X(00)00048-9
关键词
cryosections; cochlea; immunocytochemistry; in situ hybridization; hair cell; mouse
资金
- NIDCD NIH HHS [R01 DC00653] Funding Source: Medline
Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution. (C) 2001 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据