期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 20, 期 1, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.20.1.016022
关键词
animal model; inflammation; plasma leakage; fluorescence; micelle; optical imaging; fluorescence tomography
资金
- (MTA-PTE NAP B Pain Research Group) [SROP-4.2.2.A-11/1/KONV-2012-0024, KTIA_NAP_13-2014-0022, 888819, GVOP-3.2.1-2004-04-0172/3.0, PTE AOK-KA-2013/24]
- European Research Council-Belgium (Starting Independent Investigator) [206283]
- Wellcome Trust-United Kingdom (International Senior Research Fellowship) [087782]
- European Research Council (ERC) [206283] Funding Source: European Research Council (ERC)
Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of vascular leakage in diverse species using the existing small-animal fluorescence imaging technology. IR-676, a moderately hydrophobic NIR cyanine dye, was doped into self-assembling aqueous micelles using a widely employed and safe nonionic emulsifier (Kolliphor HS 15), and was tested in several acute and chronic inflammatory disease models in both mice and rats. The imaging formula is stable and exerts no acute toxic effects in vitro. It accumulated specifically in the inflamed regions in all models, which could be demonstrated by both conventional epifluorescence imaging, and fluorescence tomography both as a standalone technique and also by merging it with computed tomography scans. Ex vivo verification of dye accumulation by confocal fluorescence microscopy was also possible. The present formula allows sensitive and specific detection of inflammatory plasma leakage in diverse models. Its potential for imaging larger animals was also demonstrated. IR-676-doped micelles offer an excellent opportunity to image inflammatory vascular leakage in various models and species. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
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