期刊
JOURNAL OF CHROMATOGRAPHY B
卷 751, 期 1, 页码 199-203出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00463-1
关键词
hippuric acid; methylhippuric acid
A modified method for the simultaneous determination of hippuric acid (HA) and o-, m- and p-methylhippuric acids (o-, m- and p-MHAs) in urine is described. These metabolites were extracted, derivatized into their methyl ester derivatives and analyzed using a gas chromatograph equipped with flame ionization detector and a DB-1 capillary column. The derivatives of HA, o-, m- and p-MHAs were well separated within 11 min. The accuracy and precision in the present method were sufficient for quantitative analysis, and the results obtained by the GC method were highly correlated with those by the HPLC method (NIOSH 8301). (C) 2001 Elsevier Science BN. All rights reserved.
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