期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 20, 期 7, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.20.7.078003
关键词
multiphoton; combretastatin; spheroid; fluorescence; imaging
资金
- STFC [ST/K001914/1]
- Central Laser Facility, Rutherford Appleton Laboratory and Laserlab [EC-GA 284464]
- Science and Technology Facilities Council [ST/K001914/1] Funding Source: researchfish
The uptake of E-combretastatins, potential prodrugs of the anticancer Z-isomers, into multicellular spheroids has been imaged by intrinsic fluorescence in three dimensions using two-photon excited fluorescence lifetime imaging with 625-nm ultrafast femtosecond laser pulses. Uptake is initially observed at the spheroid periphery but extends to the spheroid core within 30 min. Using agarose gels as a three-dimensional model, the conversion of Z(trans) -> E(cis) via two-photon photoisomerization is demonstrated and the location of this photochemical process may be precisely selected within the micron scale in all three dimensions at depths up to almost 2 mm. We discuss these results for enhanced tissue penetration at longer near-infrared wavelengths for cancer therapy and up to three-photon excitation and imaging using 930-nm laser pulses with suitable combretastatin analogs. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
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