期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1841, 期 10, 页码 1483-1490出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2014.07.014
关键词
Lipid binding; Saccharomyces cerevisiae; Azole resistance; SFH3; Sterol metabolism
资金
- Scientific grant agency of the Ministry of Education of the Slovak Republic
- BBSRC
- Slovak Academy of Sciences [2/0077/10, 2/0180/12]
Pdr16p is considered a factor of clinical azole resistance in fungal pathogens. The most distinct phenotype of yeast cells lacking Pdr16p is their increased susceptibility to azole and morpholine antifungals. Pdr16p (also known as Sfh3p) of Saccharomyces cerevisiae belongs to the Sec14 family of phosphatidylinositol transfer proteins. It facilitates transfer of phosphatidylinositol (PI) between membrane compartments in in vitro systems. We generated Pdr16p(E235A,K267A) mutant defective in PI binding. This PI binding deficient mutant is not able to fulfill the role of Pdr16p in protection against azole and morpholine antifungals, providing evidence that PI binding is critical for Pdr16 function in modulation of sterol metabolism in response to these two types of antifungal drugs. A novel feature of Pdr16p, and especially of Pdr16p(E235A,K267A) mutant, to bind sterol molecules, is observed. (C) 2014 The Authors. Published by Elsevier B.V.
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