期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1841, 期 10, 页码 1422-1430出版社
ELSEVIER
DOI: 10.1016/j.bbalip.2014.06.014
关键词
Sphingolipid; Cell plate; Fatty acyl chain length; Membrane trafficking; Vesicle fusion
资金
- ANR program blanc SphingopolaR [07-BLAN-202]
- Marie Curie Fellowship FP7 [MEST-CT-2004-7576 VERT]
- Region Ile-de-France (DIM Nerf)
- Ecole des Neurosciences de Paris (ENP)
- ANR Jeunes Chercheurs (JCJC) grant [ANR-09-JCJC-0062-01]
- CGI (Commissariat General a l'Investissement)
- Conseil General de l'Essonne
- Region Ile de France
- Conseil General des Yvelines
- Agence Nationale de la Recherche (ANR) [ANR-09-JCJC-0062] Funding Source: Agence Nationale de la Recherche (ANR)
Plant cytokinesis requires intense membrane trafficking and remodeling to form a specific membrane structure, the cell plate that will ultimately separate the daughter cells. The nature and the role of lipids involved in the formation of the cell plate remain unclear. Plant membranes are particularly rich in sphingolipids such as glucosylceramides with long (16 carbons) or very long (24 carbons) acyl chains. We reveal here that inhibition of the synthesis of sphingolipids with very long acyl chains induces defective cell plates with persistent vesicular structures and large gaps. Golgi-derived vesicles carrying material toward the cell plate display longer vesicle-vesicle contact time and their cargos accumulate at the cell plate, suggesting membrane fusion and/or recycling defects. In vitro fusion experiments between artificial vesicles show that glycosphingolipids with very long acyl chains stimulate lipid bilayer fusion. Therefore we propose that the very long acyl chains of sphingolipids are essential structural determinants for vesicle dynamics and membrane fusion during cytokinesis. (C) 2014 Elsevier B.V. All rights reserved.
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