期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1841, 期 6, 页码 888-895出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2013.11.013
关键词
Lysophosphatidylcholine; Oxidation; Hepatocyte; Mitochondrion
资金
- National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health [DK069967]
- National Heart, Lung, And Blood Institute [F31HL110527]
- National Institute of General Medical Sciences [T32GM063483]
- American Heart Association [11PRE7310047]
Mice deficient in group 1b phospholipase A(2) have decreased plasma lysophosphatidylcholine and increased hepatic oxidation that is inhibited by intraperitoneal lysophosphatidylcholine injection. This study sought to identify a mechanism for lysophosphatidylcholine-mediated inhibition of hepatic oxidative function. Results showed that in vitro incubation of isolated mitochondria with 40-200 mu M lysophosphatidylcholine caused cyclosporine A-resistant swelling in a concentration-dependent manner. However, when mitochondria were challenged with 220 mu M CaCl2, cydosporine A protected against permeability transition induced by 40 mu M, but not 80 mu M lysophosphatidylcholine. Incubation with 40-120 mu M lysophosphatidylcholine also increased mitochondrial permeability to 75 mu M CaCl2 in a concentration-dependent manner. Interestingly, despite incubation with 80 mu M lysophosphatidylcholine, the mitochondrial membrane potential was steady in the presence of succinate, and oxidation rates and respiratory control indices were similar to controls in the presence of succinate, glutamate/malate, and palmitoyl-carnitine. However, mitochondrial oxidation rates were inhibited by 30-50% at 100 mu M lysophosphatidykholine. Finally, while 40 mu M lysophosphatidylcholine has no effect on fatty acid oxidation and mitochondria remained impermeable in intact hepatocytes, 100 mu M lysophosphatidylcholine inhibited fatty acid stimulated oxidation and caused intracellular mitochondrial permeability. Taken together, these present data demonstrated that LPC concentration dependently modulates mitochondrial microenvironment, with low micromolar concentrations of lysophosphatidylcholine sufficient to change hepatic oxidation rate whereas higher concentrations are required to disrupt mitochondrial integrity. (C) 2013 Published by Elsevier B.V.
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