期刊
JOURNAL OF IMMUNOLOGY
卷 166, 期 4, 页码 2235-2243出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.166.4.2235
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- NCI NIH HHS [N01-CO-56000] Funding Source: Medline
Human germinal center B cell tumors retain the ability of their nontransformed counterparts to somatically hypermutate Ig V genes by nucleotide substitution, Among a survey of 60 primary previously untreated, clonal, follicular lymphomas we have identified a rare V-H rearrangement variant and two other in-frame nucleotide insertion/deletion variants within complementarity-determining region III of the Ig heavy chain, The neoplastic origin of the V-H rearrangement variant was directly demonstrated in cells isolated by microdissection from malignant follicles. In all three cases a common clonal origin for the variants was demonstrated by complementarity-determining region III nucleotide sequence homology and shared somatic mutations in germline encoded positions in framework region IV. The monoclonal nature of the tumors was independently confirmed by demonstrating a single t(14;18) translocation breakpoint in the two cases with a detectable translocation. All the variants occurred in functional V-H rearrangements, which in two cases were directly shown to encode functional Ab molecules, Both recombination-activating genes 1 and 2 were expressed in lymph node tumor cells containing the V-H rearrangement variant, although recombination-activating gene expression among a panel of lymphomas was not limited to this variant.
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