期刊
BLOOD
卷 97, 期 4, 页码 1099-1105出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.V97.4.1099
关键词
-
类别
资金
- NHLBI NIH HHS [R01 HL61399] Funding Source: Medline
Embryonic zeta- and epsilon -globin subunits assemble with each other and with adult alpha- and beta -globin subunits into hemoglobin heterotetramers in both primitive and definitive erythrocytes, The properties of these hemoglobins-Hbs Gower-1 (zeta (2)epsilon (2)), Gower-2 (alpha (2)epsilon (2)), and Portland-2 (zeta (2)epsilon (2)), have been incompletely described as they are difficult to obtain in quantity from either primary human tissue or conventional expression systems. The generation of complex transgenic-knockout mice that express these hemoglobins at levels between 24% and 70% is described, as are efficient methods for their purification from mouse hemolysates. Key physiological characteristics-including P-50, Hill coefficient, Bohr effect, and affinity for 2,3-BPG-were established for each of the 3 human hemoglobins. The stability of each hemoglobin in the face of mechanical, thermal, and chemical stresses was also determined. Analyses indicate that the zeta -for-alpha exchange distinguishing Hb Portland-2 and Hb A alters hemoglobin O-2-transport capacity by increasing its P-50 and decreasing its Bohr effect. By comparison, the epsilon -for-beta exchange distinguishing Hb Gower-2 and Hb A has little impact on these same functional parameters. Hb Gower-1, assembled entirely from embryonic sub-units, displays an elevated P-50 level, a reduced Bohr effect, and increased 2,3-BPG binding compared to Hb A. The data support the hypothesis that Hb Gower-2, assembled from reactivated epsilon globin in individuals with defined hemoglobinopathies and thalassemias, would serve as a physiologically acceptable substitute for deficient or dysfunctional Hb A. In addition, the unexpected properties of Hb Gower-1 call into question a common hypothesis for its primary role in embryonic development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据