期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 20, 期 5, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.20.5.051038
关键词
Raman tweezers; Staphylococcus epidermidis; biofilm
资金
- Czech Science Foundation [GACR P205/11/1687]
- Ministry of Health IGA [NS-9678]
- Ministry of Education, Youth, and Sports of the Czech Republic [LO1212]
- European Commission (ALISI) [CZ.1.05/2.1.00/01.0017]
A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral Raman fingerprints obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
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