期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1525, 期 1-2, 页码 149-160出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-4165(00)00182-3
关键词
seminal fluid; carbohydrate structure; prostate-specific antigen; prostate cancer; exoglycosidase sequencing
Prostate-specific antigen (PSA) is a glycosylated chymotrypsin-like serine protease and is found mainly in prostatic tissue and seminal fluid. We purified two forms of PSA (PSA-A and PSA-B) from human seminal fluid with pI values of approx. 7.2 and approx. 6.9, respectively. To characterize the N-glycans of the two isoforms, the sugar chains were liberated by hydrazinolysis followed by N-acetylation, and derivatized with 2-aminobenzamide. Both PSA-A and PSA-B contained mono- and disialylated sugar chains, although PSA-B had a much higher content of the latter. After removal of sialic acid residues by sialidase digestion, mono- and biantennary N-glycans and three outer chain moieties (Gal beta1-4GlcNAc beta1-, GlcNAc beta1-, GalNAc beta1-4GlcNA beta1-) were found in both samples. However, the ratios of each N-glycan were different. These results indicate that PSA-A and PSA-B differ not only in their sialic acid contents, but also in their outer chain features. (C) 2001 Elsevier Science B.V. All rights reserved.
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