期刊
JOURNAL OF CHROMATOGRAPHY B
卷 751, 期 2, 页码 383-387出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00496-5
关键词
pyridoxal 5 '-phosphate; vitamin B6
Vitamin B6, measured as pyridoxal 5'-phosphate (PLP), is a co-enzyme in the transsulfuration pathway of homocysteine metabolism. Since depletion of PLP has been suggested as an independent risk factor for coronary artery disease, PLP is frequently measured to guide patient care. By a change and utilization of an Aquasil C18 column and the addition of an acetonitrile clean-up gradient to the potassium phosphate, with sodium perchlorate and bisulfite buffer between samples we report the modification of a previously described method for analysis of PLP. The result is a more practical, efficient, reliable and robust method for daily clinical use, We also determined and report that it is critical to protect freshly prepared standard PLP samples from light exposure during assay preparation. (C) 2001 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据