期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1801, 期 4, 页码 508-516出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2010.01.003
关键词
Bile salt; Digalactosyl diacylglycerol; Fatty acid; Galactolipase; Galactolipids; Gas chromatography; Mixed micelle; Monogalactosyl diacylglycerol; Pancreatic lipase-related protein 2; Thin layer chromatography
资金
- Agence Nationale de la Recherche [ANR-09-CP2D-06-01]
- CNRS
Monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the most abundant lipids in nature, mainly as important components of plant leaves and chloroplast membranes. Pancreatic lipase-related protein 2 (PLRP2) was previously found to express galactolipase activity, and it is assumed to be the main enzyme involved in the digestion of these common vegetable lipids in the gastrointestinal tract. Most of the previous in vitro studies were however performed with medium chain synthetic galactolipids as substrates. It was shown here that recombinant guinea pig (Cavia porcellus) as well as human PLRP2 hydrolyzed at high rates natural DGDG and MGDG extracted from spinach leaves Their specific activities were estimated by combining the pH-stat technique, thin layer chromatography coupled to scanning densitometry and gas chromatography The optimum assay conditions for hydrolysis of these natural long chain galactolipids were investigated and the Optimum bile salt to substrate ratio was found to be different from that established with synthetic medium chains MGDG and DGDG Nevertheless the length of acyl chains and the nature of the galactosyl polar head of the galactolipid did not have major effects on the specific activities of PLRP2, which were found to be very high on both medium chain 11786 100 to 5420 +/- 85 U/mg] and long chain [1756 208 to 4167 167 U/mg] galactolipids. Fatty acid composition analysis of natural MGDG, DGDG and their lipolysis products revealed that PLRP2 only hydrolyzed one ester bond at the sn-1 position of galactolipids. PLRP2 might be used to produce lipid and free fatty acid fractions enriched in either 16.3 n - 3 or 18:3 n - 3 fatty acids, both found at high levels in galactolipids (C) 2010 Elsevier B V. All rights reserved
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