期刊
CARBOHYDRATE RESEARCH
卷 330, 期 4, 页码 529-535出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(00)00314-1
关键词
fucoidan; fucan structures; fucan NMR; brown algae
The predominant repeating structure of a fraction of the fucoidan from Ascophyllum nodosum prepared by acid hydrolysis and centrifugal partition chromatography (LMWF) was established as: [ --> 3)-alpha -L-Fuc(2SO(3)(-))-(1 --> 4)-alpha -L-Fuc(2,3diSO(3)(-))-(1](n) by NMR spectroscopy and methylation analysis. The proton and carbon NMR spectra of this unit have been assigned and found to correspond with features in the spectra of the whole purified fucan from A. nodosum which account for most of the integrated intensity. The same structure has also been recognised in the fucoidan of Fucus vesiculosus. The fraction LMWF has in vitro anticoagulant activity, indicating that the above structure may be partly responsible for biological activity in the native fucoidan. (C) 2001 Elsevier Science Ltd. All rights reserved.
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