期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1840, 期 7, 页码 2192-2202出版社
ELSEVIER
DOI: 10.1016/j.bbagen.2014.04.005
关键词
Reactive oxygen species (ROS); Organ of Corti; Acoustic trauma; Two-photon microscopy; Laurdan; Lipid peroxidation
资金
- Fondi di Ateneo, UCSC Rome, Italy
- COST Action CM1201
Background: Noise exposure impairs outer hair cells (OHCs). The common basis for OHC dysfunction and loss by acoustic over-stimulation is represented by reactive oxygen species (ROS) overload that may affect the membrane structural organization through generation of lipid peroxidation. Methods: Here we investigated in OHC different functional zones the mechanisms linking metabolic functional state (NAD(P)H intracellular distribution) to the generation of lipid peroxides and to the physical state of membranes by two photon fluorescence microscopy. Results: In OHCs of control animals, a more oxidized NAD(P)H redox state is associated to a less fluid plasma membrane structure. Acoustic trauma induces a topologically differentiated NAD(P)H oxidation in OHC rows, which is damped between 1 and 6 h. Peroxidation occurs after similar to 4 h from noise insult, while ROS are produced in the first 0.2 h and damage cells for a period of time after noise exposure has ended (similar to 7.5 h) when a decrease of fluidity of OHC plasma membrane occurs. OHCs belonging to inner rows, characterized by a lower metabolic activity with respect to other rows, show less severe metabolic impairmerit. Conclusions: Our data indicate that plasma membrane fluidity is related to NAD(P)H redox state and lipid peroxidation in hair cells. General Significance: Our results could pave the way for therapeutic intervention targeting the onset of redox umbalance. (C) 2014 Elsevier B.V. All rights reserved.
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