期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1830, 期 12, 页码 5356-5374出版社
ELSEVIER
DOI: 10.1016/j.bbagen.2013.06.032
关键词
Human serum albumin; Structure; Oncology; Subdomain IB; Anticancer drug; Drug design
资金
- New Century Pharmaceuticals, Inc.
Background: Serum albumin is a major pharmacokinetic effector of drugs. To gain further insight into albumin binding chemistry, the crystal structures of six oncology agents were determined in complex with human serum albumin at resolutions of 2.8 to 2.0 angstrom: camptothecin, 9-amino-camptothecin, etoposide, teniposide, bicalutamide and idarubicin. Methods: Protein crystal growth and low temperature X-ray crystallography Results: These large, complex drugs are all bound within the subdomain IB binding region which can be described as a hydrophobic groove formed by alpha-helices h7, h8 and h9 covered by the extended polypeptide Ll. Ll creates a binding cavity with two access sites, one between loop Ll and alpha-helices h7 and h8 (distal site: IBd) and the other between Ll and alpha-helix h9 (proximal site: IBp). Camptothecin (2.4 angstrom) and 9 amino camptothecin (2.0 angstrom) are clearly bound as the open lactone form (IBp). Idarubicin (2.8 angstrom) binds in a DNA like dimer complex via an intermolecular pi stacking arrangement in IBd. Bicalutamide (2.4 angstrom) is bound in a folded intramolecular it stacking arrangement between two aromatic rings in IBd similar to idarubicin. Teniposide (2.7 angstrom) and etoposide (2.7 angstrom), despite small chemical differences, are bound in two distinctly different sites at or near IB. Teniposide is internalized via primarily hydrophobic interactions and spans through both openings (IBp_d). Etoposide is bound between the exterior of IB and IIA and exhibits an extensive hydrogen bonding network. Conclusions: Subdomain IB is a major binding site for complex heterocyclic molecules. General significance: The structures have important implications for drug design and development. This article is part of a Special Issue entitled Serum Albumin. (C) 2013 Elsevier B.V. All rights reserved.
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