期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1830, 期 11, 页码 5258-5266出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagen.2013.07.028
关键词
MicroRNA; MiR-181a; Salivary adenoid cystic carcinoma; Metastasis; MAPK; Snai2
资金
- National Nature Science Foundation of China [NSFC81072228, NSFC81272953]
- Guangdong Natural Science Foundation [S2011020002325]
- Research Fund for the Doctoral Program Of Ministry Of Education [20120171110050]
- Fundamental Research Funds for the Central Universities [11ykzd09]
- Program for New Century Excellent Talents in University [NCET-10-0857]
- International Cooperative Project of Science and Technology of Guangdong Province [1011420600001, 2012B031800080]
- National Institutes of Health (NIH) PHS grant [CA139596]
- T32 training grant [DE018381]
Background: To date microRNAs and their contribution to the onset and propagation of salivary adenoid cystic carcinoma (SACC) are limited. The objective of this study was to identify miR-181a and its mechanism in the metastasis of SACC. Methods: At first microarray and quantitative RT-PCR were used to investigate microRNA profiles and miR-181a in paired SACC cell lines with different metastatic potential. Then the effect of miR-181a on metastatic potential of SACC was investigated. MiR-181a target genes and Snai2 promoter activity were investigated using luciferase reporter gene assays. Western blot was used to detect MAPK-Snai2 pathway-related protein level. Results: A panel of deregulated microRNAs (including miR-181a) was identified in paired of SACC cell lines. Functional analysis indicated that miR-181a inhibited SACC cell migration, invasion and proliferation in vitro, and it suppressed tumor growth and lung metastasis in vivo. Direct targeting of miR-181a to MAP2K1, MAPK1 and Snai2 was confirmed by luciferase reporter gene assays. MiR-181a mimic inhibited the expression of MAP2K1, MAPK1 and Snai2 in SACC cells. MAP2K1 or MAPK1 siRNA suppressed Snai2 gene promoter activity and reduced Snai2 expression and the metastatic potential of SACC cells. Conclusions: Our results indicate that miR-181a plays an important role in the metastasis of SACC, and may serve as a novel therapeutic target for SACC. MiR-181a regulates the MAPK-Snai2 pathway both through direct cisregulatory mechanism and through indirect trans-regulatory mechanism. General significance: To our knowledge, this is the first study revealing that miR-181a deregulation mediated the metastasis of SACC by regulating MAPIC-Snai2 pathway. (C) 2013 Elsevier B.V. All rights reserved.
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