期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1830, 期 4, 页码 3095-3101出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagen.2012.12.028
关键词
Starch; alpha-Amylase; Binding kinetics; DSC; FTIR-ATR; Freundlich model
资金
- King's College
Background: Starch is a main source of carbohydrate in human diets, but differences are observed in postprandial glycaemia following ingestion of different foods containing identical starch contents. Such differences reflect variations in rates at which different starches are digested in the intestine. In seeking explanations for these differences, we have studied the interaction of alpha-amylase with starch granules. Understanding this key step in digestion should help with a molecular understanding for observed differences in starch digestion rates. Methods: For enzymes acting upon solid substrates, a Freundlich equation relates reaction rate to enzyme adsorption at the surface. The Freundlich exponent (n) equals 2/3 for a liquid-smooth surface interface, 1/3 for adsorption to exposed edges of ordered structures and 1.0 for solution solution. interfaces. The topography of a number of different starch granules, revealed by Freundlich exponents, was compared with structural data obtained by differential scanning calorimetiy and Fourier transform infrared spectroscopy with attenuated total internal reflectance (FTIR-ATR). Results: Enzyme binding rate and FTIR-ATR peak ratio were directly proportional to n and Delta H-gel was inversely related to n. Amylase binds fastest to solubilised starch and to granules possessing smooth surfaces at the solid liquid interface and slowest to granules possessing ordered crystalline surfaces. Conclusions: Freundlich exponents provide information about surface blocklet structures of starch that supplements knowledge obtained from physical methods. General Significance: Nanoscale structures at the surface of starch granules influence hydrolysis by alpha-amylase. This can be important in understanding how dietary starch is digested with relevance to diabetes, cardiovascular health and cancer. (C) 2013 Elsevier B.V. All rights reserved.
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