期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1830, 期 10, 页码 4500-4512出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagen.2013.05.027
关键词
Nucleolin; Heat-denatured; Human umbilical vein endothelial cells; Angiogenesis; Vascular endothelial growth factor
资金
- China National Natural Science Foundation [81071556, 81170113, 30973121]
- Clinical Specialty Major Project of Ministry of Public Health
- Natural Science Foundation of Hunan province, China [10JJ5009]
Background: The present study aims to examine the expression patterns and roles of nucleolin during the recovery of heat-denatured human umbilical vein endothelial cells (HUVECs). Methods: Deep partial thickness burn model in Sprague-Dawley rats and the heat denatured cell model (52 degrees C, 35 s) were used. The expression of nucleolin was measured using Western blot analysis and real-time PCR. Angiogenesis was assessed using in vitro parameters including endothelial cell proliferation, transwell migration assay, and scratched wound healing. Gene transfection and RNA interference approaches were employed to investigate the roles of nucleolin. Results: Nucleolin mRNA and protein expression showed a time-dependent increase during the recovery of heat-denatured dermis and HUVECs. Heat-denaturation time-dependently promoted cell growth, adhesion, migration, scratched wound healing and formation of tube-like structures in HUVECs. These effects of heat denaturation on endothelial wound healing and formation of tube-like structures were prevented by knockdown of nucleolin, whereas over-expression of nucleolin increased cell growth, migration, and formation of tube-like structures in cultured HUVEC endothelial cells. In addition, we found that the expression of vascular endothelial growth factor (VEGF) increased during the recovery of heat-denatured dermis and HUVECs, and nucleolin up-regulated VEGF in HUVECs. Conclusions: The present study reveals that the expression of nucleolin is up-regulated, and plays a pro-angiogenic role during the recovery of heat-denatured dermis and its mechanism is probably dependent on production of VEGF. General significance: We find a novel and important pro-angiogenic role of nucleolin during the recovery of heat-denatured dermis. (c) 2013 Elsevier B.V. All rights reserved.
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