期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 21, 期 6, 页码 2008-2017出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.21.6.2008-2017.2001
关键词
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资金
- NIGMS NIH HHS [R01 GM029832, GM29832] Funding Source: Medline
Expression of replication-dependent histone genes at the posttranscriptional level is controlled by stem-loop binding protein (SLBP). One function of SLBP is to bind the stem-loop structure in the 3 'untranslated region of histone pre-mRNAs and facilitate 3' end processing. Interaction of SLBP with the stem-loop is mediated by the centrally located RNA binding domain (RBD). Here we identify several highly conserved amino acids in the RED mutation of which results in complete or substantial loss of SLBP binding activity. We also identify residues in the RED which do not contribute to binding to the stem-loop RNA but instead are required for efficient recruitment of U7 snRNP to histone pre-mRNA. Recruitment of the U7 snRNP to the pre-mRNA also depends an the 20-amino-acid region located immediately downstream of the RED. A critical region of the RBD contains the sequence YDRY. The tyrosines are required for RNA binding, and the DR dipeptide is essential for processing but not for RYE binding. It is likely that the RED of SLBP interacts directly with both the stem-loop RNA and other processing factor(s), most likely the U7 snRNP, to facilitate histone pre-mRNA processing.
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