期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1810, 期 5, 页码 555-560出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagen.2011.02.003
关键词
Permeability rate; Hypothermal preservation; Cold tissue storage; Porcine aorta; Pore size
资金
- Institute of Biomedical Imaging Sciences [IBIS-97708]
- NIH/NHLBI [T32-HL07812]
- CRDF [RUB1-2932-SR-08]
- NSF [CMMI-0900743]
Background: Stored vascular tissues are employed in biomedical research for studies in imaging, in biomechanics, and/or in assessing vessel diseases. In the present study, the stability of aortic tissue in phosphate buffer saline (PBS) at 4 degrees C was monitored over a course of 10 days as determined by the rate of glucose permeation measured by optical coherence tomography (OCT) and validated by histology. Methods and results: The initial mean permeability through fresh porcine aorta was (2.32 +/- 0.46) x 10(-5) cm/s (n = 5); after maintaining the tissue at 4 degrees C for 10 days, the mean rate was (7.37 +/- 0.41) x 10(-5) cm/s (n = 4), an increase of nearly 300%. A z-test verified that a significant change in the permeability rate (p < 0.05) had occurred after 4 days of 4 degrees C storage. Histology was used to quantify changes in tissue pore area. The increase in average pore area paralleled the increase in permeability rate over 10 days. Conclusions: These results suggest that (1) the structural integrity of aortic tissue at 4 degrees C is retained for at least the first three days after resection and (2) OCT is a powerful technology well suited for evaluating tissue structural integrity over time. General significance: Functional OCT imaging provides for a noninvasive and quantitative technique in determining the structural integrity of aortic tissue stored at 4 degrees C. This modality may be used for assessing the efficacy of other preservation techniques. (C) 2011 Elsevier B.V. All rights reserved.
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