4.5 Review

Hunting for the 5th base: Techniques for analyzing DNA methylation

期刊

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
卷 1790, 期 9, 页码 847-862

出版社

ELSEVIER
DOI: 10.1016/j.bbagen.2009.02.001

关键词

DNA methylation; Maxam-Gilbert; Bisulphite sequencing; Methylation sensitive restriction enzyme; Methylated DNA immunoprecipitation; MeDIP; MSP; LUMA

资金

  1. KinderKrebsInitiative Buchholz/Holm-Seppensen
  2. Deutsche Krebshilfe [70-3173-Tr3, 107733]
  3. Bundesministerium fur Bildung und Forschung
  4. Wilhelm Sander Stiftung [2005.168.2]
  5. European Union [201444]
  6. Medical Faculty of the Christian-Albrechts University, Kiel

向作者/读者索取更多资源

Background: In the recent past large progress has been made in the analysis of the epigenome, the entirety of epigenetic modifications, and its meaning for the implementation of the genetic code. Besides histone modifications and miRNA expression, DNA methylation is one of the key players in the field of epigenetics, involved in numerous regulatory processes. Methods: In the present review we focus on methods for the analysis of DNA methylation patterns and present an overview about techniques and basic principles available for this purpose. Results and general significance: We here discuss advantages and disadvantages of various methods and their feasibility for specific tasks of DNA methylation analysis. (C) 2009 Elsevier B.V. All rights reserved.

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